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Glypican 3 Polyklonaler Antikörper

Glypican 3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30021-1-PBS

Synonyme

GPC3, DGSX, Glypican-3 alpha subunit, Glypican-3 beta subunit, GTR2 2

Filter:
  • Western Blot (WB) analysis of various lysates using Glypican 3 Polyclonal antibody (30021-1-AP)

    WB analysis using 30021-1-AP (same clone as 30021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30021-1-AP (GPC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocellular carcinoma using Glypican 3 Polyclonal antibody (30021-1-AP)

    IHC staining of human hepatocellular carcinoma using 30021-1-AP (same clone as 30021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma slide using 30021-1-AP (Glypican 3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30021-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30021-1-PBS bindet in WB, IHC, Indirect ELISA Glypican 3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Glypican 3 fusion protein Ag32559
Vollständiger Name glypican 3
Berechnetes Molekulargewicht 580 aa, 66 kDa
Beobachtetes Molekulargewicht66 kDa
GenBank-ZugangsnummerBC035972
Gene symbol GPC3
Gene ID (NCBI) 2719
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, and glycinate forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).