GPD2 Monoklonaler Antikörper

GPD2 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C12E2

Kat-Nr. : 68174-1-PBS

Synonyme

1C12E2, EC:1.1.5.3, GDH2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GPD 2

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  WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68174-1-Ig (same clone as 68174-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50432-1

  Cytometric bead array standard curve of MP50432-1, GPD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68174-2-PBS. Detection antibody: 68174-1-PBS. Standard:Ag11212. Range: 0.391-200 ng/mL.

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  Affinity and Kinetic Characterization of 68174-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 68174-1-Ig against Human GPD2 was performed. The affinity constant is 6.26 nM.

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Geprüfte Anwendungen

Produktinformation

68174-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA GPD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPD2 fusion protein Ag11212
• Vollständiger Name: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Berechnetes Molekulargewicht: 41 kDa, 81 kDa
• GenBank-Zugangsnummer: BC019874
• Gene symbol: GPD2
• Gene ID (NCBI): 2820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).