GPHN Monoklonaler Antikörper

GPHN Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D7C4

Kat-Nr. : 67995-1-PBS

Synonyme

Gephyrin, Domain E, Domain G, GEPH, GPH

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  WB analysis using 67995-1-Ig (same clone as 67995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67995-1-Ig (GPHN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67995-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67995-1-Ig (same clone as 67995-1-PBS)

  WB result of GPHN antibody (67995-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPHN transfected HEK-293 cells. This data was developed using the same antibody clone with 67995-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67995-1-PBS bindet in WB, Indirect ELISA GPHN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPHN fusion protein Ag23708
• Vollständiger Name: gephyrin
• Berechnetes Molekulargewicht: 769 aa, 83 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC030016
• Gene symbol: GPHN
• Gene ID (NCBI): 10243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.