GPI Monoklonaler Antikörper

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GPI Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F11E4

Kat-Nr. : 67178-1-PBS

Synonyme

Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9, AMF

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  WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

  WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

  WB result of GPI antibody (67178-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GPI antibody (67178-1-Ig, Clone: 2F11E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67178-1-PBS bindet in WB, IHC, IF/ICC, ELISA GPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPI fusion protein Ag7419
• Vollständiger Name: glucose phosphate isomerase
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 55-64 kDa
• GenBank-Zugangsnummer: BC004982
• Gene symbol: GPI
• Gene ID (NCBI): 2821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).