GPI Monoklonaler Antikörper

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• KD/KO Validated

GPI Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

2F11E4

Kat-Nr. : CL594-67178

Synonyme

AMF, Autocrine motility factor, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36

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  IF Staining of PC-3 using CL594-67178

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite® Plus 594 GPI antibody (CL594-67178, Clone: 2F11E4 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67178 bindet in IF/ICC GPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPI fusion protein Ag7419
• Vollständiger Name: glucose phosphate isomerase
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 55-64 kDa
• GenBank-Zugangsnummer: BC004982
• Gene symbol: GPI
• Gene ID (NCBI): 2821
• Konjugation: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 594 nm / 615 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).