GPNMB Polyklonaler Antikörper

GPNMB Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27037-1-AP

Synonyme

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

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  WB analysis using 27037-1-AP

  TT cells were subjected to SDS PAGE followed by western blot with 27037-1-AP (GPNMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L-929 using 27037-1-AP

  L-929 cells were subjected to SDS PAGE followed by western blot with 27037-1-AP (GPNMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: TT cells, L-929-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27037-1-AP bindet in WB, ELISA GPNMB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPNMB fusion protein Ag25826
• Vollständiger Name: glycoprotein (transmembrane) nmb
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 95 kDa
• GenBank-Zugangsnummer: BC011595
• Gene symbol: GPNMB
• Gene ID (NCBI): 10457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.