GPNMB Monoklonaler Antikörper

GPNMB Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

CloneNo.

2B10B8

Kat-Nr. : 66926-1-Ig

Synonyme

2B10B8, Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSK-BR-3 cells, 4T1-Zellen, A375-Zellen, A549-Zellen, HeLa-Zellen, HT-1080.Zellen, MDA-MB-468-Zellen, PC-12-Zellen, RAW 264.7-Zellen, THP-1-Zellen, U-251-Zellen, U-937-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66926-1-Ig bindet in WB, IHC, IF/ICC, ELISA GPNMB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GPNMB fusion protein Ag26747
Vollständiger Name glycoprotein (transmembrane) nmb
Berechnetes Molekulargewicht 64 kDa
Beobachtetes Molekulargewicht 64-70 kDa
GenBank-ZugangsnummerBC011595
Gene symbol GPNMB
Gene ID (NCBI) 10457
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GPNMB antibody 66926-1-IgProtokoll herunterladen
IF protocol for GPNMB antibody 66926-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC,IF

Int Immunopharmacol

Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma

Authors - Ying Lin
WB

PLoS Negl Trop Dis

Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach

Authors - Yajie Yuan
human

medRxiv

Plasticity of Human Microglia and Brain Perivascular Macrophages in Aging and Alzheimer's Disease

Authors - Donghoon Lee
mouseWB

Hum Vaccin Immunother

Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction

Authors - Kexin Zhang
humanIF

Immunity

Direct microglia replacement reveals pathologic and therapeutic contributions of brain macrophages to a monogenic neurological disease

Authors - William H Aisenberg
humanIHC

Cancers (Basel)

TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma

Authors - Soji Toda

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Victor (Verified Customer) (06-16-2025)

Staining with the GPNMB antibody on myeloid cells using the recommended concentration for immunofluorescence is highly specific. The signal shows minimal background noise and appears very clear and distinct.

  • Applications: Western Blot, Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500 as recommended
  • Cell Tissue Type: Macrophages
GPNMB Antibody Western Blot,Immunofluorescence,Cell culture validation (1:500 as recommended dilution) in Macrophages (Cat no:66926-1-Ig)