GPNMB Rekombinanter Antikörper

GPNMB Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241435F3

Kat-Nr. : 84160-3-RR

Synonyme

241435F3, Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB

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  WB analysis using 84160-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84160-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 84160-3-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84160-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (84160-3-RR, Clone: 241435F3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84160-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84160-3-RR against Human GPNMB were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84160-3-RR bindet in WB, IF/ICC, ELISA GPNMB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GPNMB fusion protein Ag14124
• Vollständiger Name: glycoprotein (transmembrane) nmb
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 95 kDa
• GenBank-Zugangsnummer: BC011595
• Gene symbol: GPNMB
• Gene ID (NCBI): 10457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.