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GPNMB Monoklonaler Antikörper

GPNMB Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2B10B8

Kat-Nr. : CL594-66926

Synonyme

GPNMB, HGFIN, Transmembrane glycoprotein NMB

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GPNMB Monoclonal antibody (CL594-66926)

    IF Staining of HeLa using CL594-66926

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 GPNMB antibody (CL594-66926, Clone: 2B10B8 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66926 bindet in IF GPNMB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GPNMB fusion protein Ag26747
Vollständiger Name glycoprotein (transmembrane) nmb
Berechnetes Molekulargewicht 64 kDa
GenBank-ZugangsnummerBC011595
Gene symbol GPNMB
Gene ID (NCBI) 10457
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 GPNMB antibody CL594-66926Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen