GPR107 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GPR107 Polyklonal Antikörper für FC, IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 25076-1-AP

Synonyme

G protein coupled receptor 107, GCDRP, GPR107, KIAA1624, LUSTR1, Protein GPR107, RP11 88G17

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 25076-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25076-1-AP (GPR107 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 25076-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25076-1-AP (GPR107 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 25076-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25076-1-AP (GPR107 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of SH-SY5Y using 25076-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GPR107 antibody (25076-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

25076-1-AP bindet in WB, IHC, IF, FC, ELISA GPR107 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR107 fusion protein Ag18649
• Vollständiger Name: G protein-coupled receptor 107
• Berechnetes Molekulargewicht: 552 aa, 67 kDa
• Beobachtetes Molekulargewicht: 60-67 kDa
• GenBank-Zugangsnummer: BC110518
• Gene symbol: GPR107
• Gene ID (NCBI): 57720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR107 also named as KIAA1624 or LUSTR1 is 600 amino acid protein, which belongs to the LU7TM family. GPR107 localizes in membrane and is a multi-pass membrane protein. GPR107 is a promising candidate receptor for neuronostatin, and neuronostatin, interacting with GPR107, may play an important role in the central control of cardiovascular function.

Publikationen

Species	Application	Title
mouse	WB	Nat Chem Biol; A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.; Authors - Yu-Shiuan Cheng
human	WB,IHC,IF	J Exp Clin Cancer Res; LncGPR107 drives the self-renewal of liver tumor initiating cells and liver tumorigenesis through GPR107-dependent manner.; Authors - Guanqun Huang
mouse	WB	Neurochem Res; Neuronostatin Promotion Soluble Aβ1-42 Oligomers: Induced Dysfunctional Brain Glucose Metabolism in Mice.; Authors - Shaobin Yang
mouse	WB	Behav Brain Res; Neuronostatin promotes soluble Aβ1-42 oligomers -induced spatial learning and memory impairments in mice.; Authors - Shaobin Yang ; KD Validated
human	WB	Nat Chem Biol; Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A; Authors - Xiaohui Zhao