GPR155 Polyklonaler Antikörper

GPR155 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12659-1-PBS

Synonyme

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  WB analysis using 12659-1-AP (same clone as 12659-1-PBS)

  mouse brain lysates were subjected to SDS PAGE followed by western blot with 12659-1-AP (GPR155 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

  IP result of anti-GPR155 (IP:12659-1-AP, 4ug; Detection:12659-1-AP 1:300) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12659-1-PBS bindet in WB, IHC, IP, Indirect ELISA GPR155 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR155 fusion protein Ag3328
• Vollständiger Name: G protein-coupled receptor 155
• Berechnetes Molekulargewicht: 870 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa, 75 kDa
• GenBank-Zugangsnummer: BC028730
• Gene symbol: GPR155
• Gene ID (NCBI): 151556
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The G Protein-coupled receptor 155 (GPR155), also known as Lysosomal cholesterol signaling protein (LYCHOS), is a cholesterol receptor on the lysosome. GRR155 plays a role in the cholesterol-sensing lysosomal pathway and couples cholesterol concentration to MTORC1-dependent anabolic signaling (PMID: 36007018). GPR155 may represent a biomarker for diagnosing and predicting hematogenous metastasis of gastric cancer (PMID: 28165032).