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GPR161 Polyklonaler Antikörper

GPR161 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29328-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using GPR161 Polyclonal antibody (29328-1-AP)

    WB analysis using 29328-1-AP (same clone as 29328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29328-1-AP (GPR161 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using GPR161 Polyclonal antibody (29328-1-AP)

    IF Staining of ARPE-19 using 29328-1-AP (same clone as 29328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using GPR161 Polyclonal antibody (29328-1-AP)

    IF Staining of hTERT-RPE1 using 29328-1-AP (same clone as 29328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using GPR161 Polyclonal antibody (29328-1-AP)

    IF Staining of ARPE-19 using 29328-1-AP (same clone as 29328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29328-1-PBS bindet in WB, IF/ICC, Indirect ELISA GPR161 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GPR161 fusion protein Ag30998
Vollständiger Name G protein-coupled receptor 161
Berechnetes Molekulargewicht 529 aa, 59 kDa
Beobachtetes Molekulargewicht70 kDa
GenBank-ZugangsnummerBC028163
Gene symbol GPR161
Gene ID (NCBI) 23432
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band detected by this antibody probably represents a modified version of GPR161 (PMID: 18250320).