GPR172B Polyklonaler Antikörper

GPR172B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20791-1-PBS

Synonyme

RFT1, Protein GPR172B, PERV A receptor 2, PAR2, hRFT1

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  WB analysis using 20791-1-AP (same clone as 20791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20791-1-AP (GPR172B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20791-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 20791-1-AP (same clone as 20791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20791-1-AP (GPR172B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20791-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20791-1-PBS bindet in WB, IHC, Indirect ELISA GPR172B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR172B fusion protein Ag14525
• Vollständiger Name: G protein-coupled receptor 172B
• Berechnetes Molekulargewicht: 448 aa, 46 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC060810
• Gene symbol: GPR172B
• Gene ID (NCBI): 55065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR172B, also known as solute carrier family 52 member 1 (SLC52A1) or riboflavin transporter 1 (RFT1), is a G protein-coupled receptor (GPCR) that plays a significant role in the transport of riboflavin, a vital component for the production of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). GPR172B plays a role in cellular aging. Knockdown of GPR172B promotes senescence induced by DNA damage in both tumor and normal cells. The anti-senescence effect of GPR172B is dependent on its riboflavin transport activity, which leads to the activation of mitochondrial membrane potential (MMP) mediated by mitochondrial electron transport chain complex II, ultimately inhibiting the AMPK-p53 pathway, a central mediator of senescence-associated with mitochondrial dysfunction.