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GPR175 Polyklonaler Antikörper

GPR175 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33499-1-PBS

Synonyme

Integral membrane protein GPR175, PP6566, TMEM227, TPRA1, TPRA40

Filter:
  • Western Blot (WB) analysis of various lysates using GPR175 Polyclonal antibody (33499-1-AP)

    WB analysis using 33499-1-AP (same clone as 33499-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 33499-1-AP (GPR175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33499-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPR175 Polyclonal antibody (33499-1-AP)

    WB analysis using 33499-1-AP (same clone as 33499-1-PBS)

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 33499-1-AP (GPR175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33499-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

33499-1-PBS bindet in WB, Indirect ELISA GPR175 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GPR175 fusion protein Ag39379
Vollständiger Name G protein-coupled receptor 175
Beobachtetes Molekulargewicht55 kDa
GenBank-ZugangsnummerBC050711
Gene symbol GPR175
Gene ID (NCBI) 131601
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR175, also known as TPRA1 or TPRA40, is a transmembrane protein. GPR175 was first cloned as a GLP-1 receptor homolog in 3T3-L1 adipocytes and is also expressed in tissues that require Hh signaling during development, including the heart, brain, lung, pancreas, and muscle. Endogenous GPR175 localizes to cilia in a Hh-dependent manner, where it can interact with (constitutively) ciliary Gαi1 to inhibit the local production of cAMP by adenylyl cyclase. The calculated molecular weight of GPR175 is 41 kDa. With glycosylation modification, the molecular weight of GPR175 will be migrated to 55 kDa. (PMID: 26451044)