GPR183/EBI2 Polyklonaler Antikörper

GPR183/EBI2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12377-1-AP

Synonyme

EBI2, G protein coupled receptor 183, GP183, GPR183

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 12377-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12377-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12377-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12377-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12377-1-AP

  IP Result of anti-GPR183/EBI2 (IP:12377-1-AP, 4ug; Detection:12377-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Mauslungengewebe, Raji-Zellen
• Erfolgreiche IP: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12377-1-AP bindet in WB, IP, ELISA GPR183/EBI2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR183/EBI2 fusion protein Ag3035
• Vollständiger Name: G protein-coupled receptor 183
• Berechnetes Molekulargewicht: 361 aa, 41 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC020752
• Gene symbol: GPR183
• Gene ID (NCBI): 1880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Allergy Clin Immunol; Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.; Authors - Hulse Kathryn E KE
human	WB	J Pharmacol Exp Ther; 7α,25-Dihydroxycholesterol Suppresses Hepatocellular Steatosis through GPR183/EBI2 in Mouse and Human Hepatocytes.; Authors - Jin Huang
mouse	WB	Neurotox Res; Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome.; Authors - Xiangliang Zeng
mouse	WB	Eur J Pharmacol; Activation of G-protein-coupled receptor 183 initiates inflammatory pain via macrophage CCL22 secretion; Authors - Zhenhua Qi