GPR183/EBI2 Polyklonaler Antikörper

GPR183/EBI2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12377-1-AP

Synonyme

EBI2, GPR183, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2, G protein coupled receptor 183

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  WB analysis of Jurkat using 12377-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12377-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12377-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12377-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12377-1-AP

  IP result of anti-GPR183/EBI2 (IP:12377-1-AP, 4ug; Detection:12377-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse brain using 12377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12377-1-AP (GPR183/EBI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Mauslungengewebe, Raji-Zellen
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

12377-1-AP bindet in WB, IHC, IP, ELISA GPR183/EBI2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR183/EBI2 fusion protein Ag3035
• Vollständiger Name: G protein-coupled receptor 183
• Berechnetes Molekulargewicht: 361 aa, 41 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC020752
• Gene symbol: EBI2
• Gene ID (NCBI): 1880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G-protein-coupled receptor 183, also known as Epstein-Barr virusinduced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).

Publikationen

Species	Application	Title
human	WB	J Allergy Clin Immunol; Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.; Authors - Hulse Kathryn E KE
human	WB	J Pharmacol Exp Ther; 7α,25-Dihydroxycholesterol Suppresses Hepatocellular Steatosis through GPR183/EBI2 in Mouse and Human Hepatocytes.; Authors - Jin Huang
mouse	WB	Neurotox Res; Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome.; Authors - Xiangliang Zeng
mouse	WB	Eur J Pharmacol; Activation of G-protein-coupled receptor 183 initiates inflammatory pain via macrophage CCL22 secretion; Authors - Zhenhua Qi
mouse	WB	Br J Nutr; RNA-seq screening and gene function analysis uncover GPR183 as a key mediator for methionine to stimulate milk synthesis in mouse mammary epithelial cells; Authors - Yuwen Zhou