GPR35 Polyklonaler Antikörper

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• KD/KO Validated

GPR35 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 55248-1-AP

Synonyme

G protein coupled receptor 35, GPR35, KYNA receptor, Kynurenic acid receptor

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Galerie der Validierungsdaten

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  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55248-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 55248-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 55248-1-AP

  1X10^6 A549 cells were stained with 0.2ug GPR35 antibody (55248-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Hirngewebe, humanes Magengewebe
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

55248-1-AP bindet in WB, FC, ELISA GPR35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: G protein-coupled receptor 35
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: NM_005301
• Gene symbol: GPR35
• Gene ID (NCBI): 2859
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR35, also named as KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

Publikationen

Species	Application	Title
human	WB	Acta Pharm Sin B; Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage.; Authors - Di Wang ; KD Validated
human	WB	Eur J Pharmacol; Aminosalicylates target GPR35, partly contributing to the prevention of DSS-induced colitis; Authors - Wuxiyar Otkur