GPR37/Pael-R Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GPR37/Pael-R Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14820-1-PBS

Synonyme

GPR37, Pael-R, EDNRBL, Endothelin B receptor-like protein 1, ETBR LP 1

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  WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14820-1-AP (same clone as 14820-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14820-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA GPR37/Pael-R und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR37/Pael-R fusion protein Ag6591
• Vollständiger Name: G protein-coupled receptor 37 (endothelin receptor type B-like)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC040007
• Gene symbol: GPR37
• Gene ID (NCBI): 2861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).