GPR81 Polyklonaler Antikörper

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• KD/KO Validated

GPR81 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20146-1-PBS

Synonyme

FKSG80, G protein coupled receptor 104, G protein coupled receptor 81, GPR104, G-protein coupled receptor 104

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  WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20146-1-AP (GPR81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 20146-1-AP (same clone as 20146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20146-1-PBS bindet in WB, IHC, Indirect ELISA GPR81 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR81 fusion protein Ag13682
• Vollständiger Name: G protein-coupled receptor 81
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC066881
• Gene symbol: GPR81
• Gene ID (NCBI): 27198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR81 (G protein-coupled receptor 81) is one of a large family of GPRs with low affinity for hydroxy-carboxylic acid structure ligands (PMID: 24657625). Lactate functions as a signaling molecule by serving as an agonist for the GPR81, involving both autocrine and paracrine mechanisms (PMID: 31836453). Moreover, A higher molecular mass band (∼60 kDa) is present in the brain and adipose tissue. This band is also strong in the transfected HeLa cells but weak in native HeLa cells and is therefore probably a form of GPR81, possibly a glycosylated form of the receptor (PMID: 23696276).