GPRC5A,RAI3 Polyklonaler Antikörper

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• KD/KO Validated

GPRC5A,RAI3 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 10309-1-AP

Synonyme

GPRC5A, GPCR5A, G-protein coupled receptor family C group 5 member A, PEIG-1, Phorbol ester induced gene 1

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  WB analysis using 10309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10309-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung squamous cell carcinoma using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HeLa using 10309-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug GPRC5A,RAI3 Polyclonal antibody (10309-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, MCF-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10309-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA GPRC5A,RAI3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPRC5A,RAI3 fusion protein Ag0311
• Vollständiger Name: G protein-coupled receptor, family C, group 5, member A
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC003665
• Gene symbol: GPRC5A
• Gene ID (NCBI): 9052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Publikationen

Species	Application	Title
goat	IHC	Cell Prolif; Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis; Authors - Peng Lin
human	WB	Sci Total Environ; GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.; Authors - Ziling Huang ; KO Validated
Human	WB	Biol Open; Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer.; Authors - Guoqiang Wang
human	WB	Oncol Lett; Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.; Authors - Er Jin
human	WB	Exp Lung Res; GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.; Authors - Jin Er
		Mol Med Rep; Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.; Authors - Er Jin

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