GPSM1 Monoklonaler Antikörper

GPSM1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A7A2

Kat-Nr. : 68233-1-PBS

Synonyme

AGS3, DKFZp727I051, GPSM1

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  WB analysis using 68233-1-Ig (same clone as 68233-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68233-1-Ig (GPSM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68233-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 68233-1-Ig (same clone as 68233-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using GPSM1 antibody (68233-1-Ig, Clone: 3A7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68233-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68233-1-PBS bindet in WB, IF/ICC, Indirect ELISA GPSM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPSM1 fusion protein Ag18773
• Vollständiger Name: G-protein signaling modulator 1 (AGS3-like, C. elegans)
• Berechnetes Molekulargewicht: 652 aa, 72 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC017353
• Gene symbol: GPSM1
• Gene ID (NCBI): 26086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).