GPSM2 Polyklonaler Antikörper

GPSM2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26798-1-AP

Synonyme

GPSM2, LGN, Mosaic protein LGN, Pins

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  WB analysis using 26798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26798-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26798-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26798-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 26798-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 26798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 26798-1-AP (GPSM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HEK-293T-Zellen, Mausherzgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

26798-1-AP bindet in WB, IHC, IF/ICC, ELISA GPSM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPSM2 fusion protein Ag25195
• Vollständiger Name: G-protein signaling modulator 2 (AGS3-like, C. elegans)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70-77 kDa
• GenBank-Zugangsnummer: BC027732
• Gene symbol: GPSM2
• Gene ID (NCBI): 29899
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; Comprehensive Analysis of GPSM2: From Pan-Cancer Analysis to Experimental Validation; Authors - Chunjiao Yang
human	IHC	Nan Fang Yi Ke Da Xue Xue Bao; [GPSM2 is highly expressed in gastric cancer to affect patient prognosis by promoting tumor cell proliferation]; Authors - Xue Song