GPX3 Polyklonaler Antikörper

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• KD/KO Validated

GPX3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 13947-1-AP

Synonyme

Glutathione peroxidase 3, GPx 3, GPx P, GPX3, GPXP, GSHPx 3, GSHPx P, Plasma glutathione peroxidase

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 13947-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13947-1-AP (GPX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat eye using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat eye using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13947-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GPX3 antibody (13947-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Ratten-Augengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

13947-1-AP bindet in WB, IHC, IF, ELISA GPX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPX3 fusion protein Ag5054
• Vollständiger Name: glutathione peroxidase 3 (plasma)
• Berechnetes Molekulargewicht: 226 aa, 26 kDa
• Beobachtetes Molekulargewicht: 20-27 kDa
• GenBank-Zugangsnummer: BC050378
• Gene symbol: GPX3
• Gene ID (NCBI): 2878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).

Publikationen

Species	Application	Title
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
rat	WB	Oxid Med Cell Longev; The Secretome Deregulations in a Rat Model of Endotoxemic Shock.; Authors - A Blangy-Letheule
mouse	WB	Bioorg Chem; Two resveratrol analogs, pinosylvin and 4,4'-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway.; Authors - Cheng-Niu Wang
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan
chicken	WB	J Cell Physiol; Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes.; Authors - Yafan Gong ; KD Validated