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GPX4 Monoklonaler Antikörper

GPX4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratte, Zebrafisch und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(697)

CloneNo.

3F5G5

Kat-Nr. : 67763-1-Ig

Synonyme

3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBEC109-Zellen, 4T1-Zellen, ATDC-5-Zellen, Hühnerhirngewebe, CHO-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Thrombozytengewebe, Jurkat-Zellen, MDCK-Zellen, Maushirngewebe, Maushodengewebe, NCCIT-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Kaninchenhodengewebe, Rattenhirngewebe, Zebrafischgewebe
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, Maushodengewebe, Rattenhodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushodengewebe
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67763-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA GPX4 und zeigt Reaktivität mit hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratten, Zebrafisch

Getestete Reaktivität hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratte, Zebrafisch
In Publikationen genannte Reaktivitäthuman, Ente, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Rind, Ziege
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen GPX4 fusion protein Ag30650
Vollständiger Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Beobachtetes Molekulargewicht 20-23 kDa
GenBank-ZugangsnummerBC021567
Gene symbol GPX4
Gene ID (NCBI) 2879
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GPX4 antibody 67763-1-IgProtokoll herunterladen
IHC protocol for GPX4 antibody 67763-1-IgProtokoll herunterladenl
IF protocol for GPX4 antibody 67763-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Host Microbe

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Authors - Yunong Zeng
mouse,humanWB

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
ratIF

Redox Biol

LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX

Authors - Dongya Jiang
humanWB,IHC

Redox Biol

Targeting GPX4-mediated ferroptosis protection sensitizes BRCA1-deficient cancer cells to PARP inhibitors

Authors - Xuexia Xie
mouseWB

Redox Biol

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease

Authors - Yi Sun
mouseWB

Drug Des Devel Ther

Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides

Authors - Xuedong Yin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Pooja (Verified Customer) (09-08-2025)

mouse retina paraffin embedded section incubated with the GPx4 antibody (1:100 dilution) at 4 degree overnight.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse retina
GPX4 Antibody Immunohistochemistry validation (1:100 dilution) in Mouse retina (Cat no:67763-1-Ig)
FH

Breanna (Verified Customer) (08-11-2025)

The antibody worked well when testing my GPX4 KO cells! Highly recommend.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Rajeshkumar (Verified Customer) (07-23-2025)

works very good for human and mic lysates

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: 1-1000
  • Cell Tissue Type: Human and mouse
FH

Vasu (Verified Customer) (09-23-2024)

We evaluated antibodies from various manufacturers but failed to achieve satisfactory results. However, using the ProteinTech antibody, we successfully detected robust and specific bands corresponding to the expected protein size.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Breast Cancer and other cancer cells and tumor tissue samples
FH

Tianyi (Verified Customer) (08-17-2023)

Paraffine-embedded ovarian tissue (4% PFA, 24 h) incubated with GPX4 (1:200 dilution) and secondary antibody Alexa fluor donkey anti-mouse 647 IgG (H+L).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Ovarian tissue
GPX4 Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:67763-1-Ig)