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GPX4 Monoklonaler Antikörper
GPX4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratte, Zebrafisch und mehr (3)
Anwendung
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
3F5G5
Kat-Nr. : 67763-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | EC109-Zellen, 4T1-Zellen, ATDC-5-Zellen, Hühnerhirngewebe, CHO-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Thrombozytengewebe, Jurkat-Zellen, MDCK-Zellen, Maushirngewebe, Maushodengewebe, NCCIT-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Kaninchenhodengewebe, Rattenhirngewebe, Zebrafischgewebe |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, Maushodengewebe, Rattenhodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Maushodengewebe |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 7 publications below |
WB | See 633 publications below |
IHC | See 132 publications below |
IF | See 116 publications below |
IP | See 9 publications below |
CoIP | See 4 publications below |
Produktinformation
67763-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA GPX4 und zeigt Reaktivität mit hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratten, Zebrafisch
Getestete Reaktivität | hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratte, Zebrafisch |
In Publikationen genannte Reaktivität | human, Ente, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Rind, Ziege |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | GPX4 fusion protein Ag30650 |
Vollständiger Name | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
Beobachtetes Molekulargewicht | 20-23 kDa |
GenBank-Zugangsnummer | BC021567 |
Gene symbol | GPX4 |
Gene ID (NCBI) | 2879 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for GPX4 antibody 67763-1-Ig | Protokoll herunterladen |
IHC protocol for GPX4 antibody 67763-1-Ig | Protokoll herunterladenl |
IF protocol for GPX4 antibody 67763-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Host Microbe Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice | ||
Nucleic Acids Res MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation | ||
Redox Biol LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX | ||
Redox Biol Targeting GPX4-mediated ferroptosis protection sensitizes BRCA1-deficient cancer cells to PARP inhibitors | ||
Redox Biol A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease | ||
Drug Des Devel Ther Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Pooja (Verified Customer) (09-08-2025) | mouse retina paraffin embedded section incubated with the GPx4 antibody (1:100 dilution) at 4 degree overnight.
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FH Breanna (Verified Customer) (08-11-2025) | The antibody worked well when testing my GPX4 KO cells! Highly recommend.
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FH Rajeshkumar (Verified Customer) (07-23-2025) | works very good for human and mic lysates
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FH Vasu (Verified Customer) (09-23-2024) | We evaluated antibodies from various manufacturers but failed to achieve satisfactory results. However, using the ProteinTech antibody, we successfully detected robust and specific bands corresponding to the expected protein size.
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FH Tianyi (Verified Customer) (08-17-2023) | Paraffine-embedded ovarian tissue (4% PFA, 24 h) incubated with GPX4 (1:200 dilution) and secondary antibody Alexa fluor donkey anti-mouse 647 IgG (H+L).
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