GPX4 Monoklonaler Antikörper

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• KD/KO Validated

GPX4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F5G5

Kat-Nr. : 67763-1-PBS

Synonyme

3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4

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  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67763-1-Ig (same clone as 67763-1-PBS)

  WB result of GPX4 antibody (67763-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67763-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA GPX4 und zeigt Reaktivität mit hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: hamster, Hausschwein, Huhn, human, hund, Kaninchen, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPX4 fusion protein Ag30650
• Vollständiger Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC021567
• Gene symbol: GPX4
• Gene ID (NCBI): 2879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.