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GPX4 (Human Specific) Rekombinanter Antikörper

GPX4 (Human Specific) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6E9

Kat-Nr. : 82822-2-PBS

Synonyme

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

Filter:
  • Western Blot (WB) analysis of various lysates using GPX4 (Human Specific) Recombinant antibody (82822-2-RR)

    WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GPX4 (Human Specific) Recombinant antibody (82822-2-RR)

    WB analysis of HEK-293 using 82822-2-RR (same clone as 82822-2-PBS)

    WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82822-2-RR

    Affinity and Kinetic Characterization of 82822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

82822-2-PBS bindet in WB, Indirect ELISA GPX4 (Human Specific) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GPX4 (Human Specific) fusion protein Ag30650
Vollständiger Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Beobachtetes Molekulargewicht 20-23 kDa
GenBank-ZugangsnummerBC021567
Gene symbol GPX4
Gene ID (NCBI) 2879
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.