GPX5 Polyklonaler Antikörper

GPX5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18731-1-PBS

Synonyme

GSHPx 5, GPx-5, GPx 5, Epididymis-specific glutathione peroxidase-like protein, EGLP

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  WB analysis of mouse testis using 18731-1-AP (same clone as 18731-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 18731-1-AP (same clone as 18731-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18731-1-AP (same clone as 18731-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18731-1-PBS bindet in WB, IHC, Indirect ELISA GPX5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glutathione peroxidase 5 (epididymal androgen-related protein)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 22-28 kDa
• GenBank-Zugangsnummer: NM_001509
• Gene symbol: GPX5
• Gene ID (NCBI): 2880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.