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GR repeat Polyklonaler Antikörper
GR repeat Polyklonal Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IHC, IF, Dot Blot, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23978-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Recombinant protein |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IHC | See 7 publications below |
| IF | See 11 publications below |
Produktinformation
23978-1-AP bindet in WB, IHC, IF, Dot Blot, ELISA GR repeat und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, drosophila, Maus, Zebrafisch |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | GR repeat |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence "GRGRGRGRGRGRGRGRGR" were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for GR repeat antibody 23978-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Science C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins. | ||
Nat Neurosci RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
