GR repeat Polyklonaler Antikörper

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• KD/KO Validated

GR repeat Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, Dot Blot, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 23978-1-AP

Synonyme

C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 23978-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 23978-1-AP (GR repeat Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 8 publications below

Produktinformation

23978-1-AP bindet in WB, IHC, IF, Dot Blot, ELISA GR repeat und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, drosophila, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GR repeat
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence "GRGRGRGRGRGRGRGRGR" were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.

Publikationen

Species	Application	Title
	WB	Nature; The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.; Authors - Ke Zhang
Drosophila	WB	Science; C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins.; Authors - Sarah Mizielinska
human	IF	Cell; Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.; Authors - Ke Zhang ; KD Validated
human	IHC	Nat Neurosci; RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.; Authors - Shizuka B Yamada
zebrafish	Dot Blot	Acta Neuropathol; A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism.; Authors - Bart Swinnen
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku

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