GRB10 Polyklonaler Antikörper

GRB10 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 23591-1-AP

Synonyme

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS

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  WB analysis using 23591-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23591-1-AP (GRB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23591-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23591-1-AP (GRB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 23591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23591-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23591-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23591-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRB10 antibody (23591-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below

Produktinformation

23591-1-AP bindet in WB, IHC, IF/ICC, ELISA GRB10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRB10 fusion protein Ag20355
• Vollständiger Name: growth factor receptor-bound protein 10
• Berechnetes Molekulargewicht: 536aa,61 kDa; 594aa,67 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC024285
• Gene symbol: GRB10
• Gene ID (NCBI): 2887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation.

Publikationen

Species	Application	Title
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	WB	J Clin Invest; TSC1 regulates the balance between effector and regulatory T cells.; Authors - Park Yoon Y
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang
human	WB	Cancer Cell Int; GRB10 is a novel oncogene associated with cell proliferation and prognosis in glioma.; Authors - Yuanbing Chen
human	WB,IHC	Reproduction; MiR199a is implicated in embryo implantation by regulating Grb10 in rat.; Authors - Xia Hong-Fei HF
human	WB	DNA Cell Biol; Modulation of IGF1R Signaling Pathway by GIGYF1 in High Glucose-Induced SHSY-5Y Cells.; Authors - Ting Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody detects a specific band of ~70 kDa, corresponding to predicted molecular weight of GRB10.

• Applications: Western Blot
• Cell Tissue Type: Heart