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GRB10 Rekombinanter Antikörper

GRB10 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D13

Kat-Nr. : 81225-1-PBS

Synonyme

KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13

Filter:
  • Western Blot (WB) analysis of various lysates using GRB10 Recombinant antibody (81225-1-RR)

    WB analysis using 81225-1-RR (same clone as 81225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81225-1-RR (GRB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GRB10 Recombinant antibody (81225-1-RR)

    IF Staining of LNCaP using 81225-1-RR (same clone as 81225-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using GRB10 antibody (81225-1-RR, Clone: 1D13 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81225-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81225-1-RR

    Affinity and Kinetic Characterization of 81225-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81225-1-RR against Human GRB10 were performed. The affinity constant is 0.195 nM.

Geprüfte Anwendungen

Produktinformation

81225-1-PBS bindet in WB, IF/ICC, Indirect ELISA GRB10 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GRB10 fusion protein Ag20355
Vollständiger Name growth factor receptor-bound protein 10
Berechnetes Molekulargewicht536aa,61 kDa; 594aa,67 kDa
Beobachtetes Molekulargewicht60-70 kDa
GenBank-ZugangsnummerBC024285
Gene symbol GRB10
Gene ID (NCBI) 2887
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.