GRB2 Polyklonaler Antikörper

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• KD/KO Validated

GRB2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 10254-2-AP

Synonyme

Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10254-2-AP

  WB result of GRB2 antibody (10254-2-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10254-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10254-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 10254-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10254-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IHC staining of human pancreas cancer using 10254-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10254-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10254-2-AP

  Immunofluorescent analysis of HEK-293 cells using 10254-2-AP (GRB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, Rattenhirngewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

10254-2-AP bindet in WB, IHC, IF, ELISA GRB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRB2 fusion protein Ag0395
• Vollständiger Name: growth factor receptor-bound protein 2
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC000631
• Gene symbol: GRB2
• Gene ID (NCBI): 2885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Publikationen

Species	Application	Title
human	IHC,WB	Mol Ther Nucleic Acids; ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.; Authors - Lingjiao Meng
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	IHC	Cancer Lett; Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2.; Authors - Jie Lv
human	WB,IHC	Oncotarget; mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.; Authors - Xinjing Wang
		Cancer Lett; Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma.; Authors - Xinjing Wang
human	WB	Cancer Sci; In vivo CRISPR screens identify RhoV as a pro-metastasis factor of triple-negative breast cancer; Authors - Ming-Liang Jin