GRB2 Monoklonaler Antikörper

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GRB2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C5D11

Kat-Nr. : 66880-1-PBS

Synonyme

1C5D11, ASH, EGFRBP GRB2, Grb33, Growth factor receptor-bound protein 2

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  WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66880-1-Ig (same clone as 66880-1-PBS)

  WB result of GRB2 antibody (66880-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66880-1-PBS bindet in WB, IHC, ELISA GRB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRB2 fusion protein Ag0395
• Vollständiger Name: growth factor receptor-bound protein 2
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC000631
• Gene symbol: GRB2
• Gene ID (NCBI): 2885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.