GRB7 Polyklonaler Antikörper

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• KD/KO Validated

GRB7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10045-1-PBS

Synonyme

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  WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10045-1-Ig (same clone as 10045-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  IP result of anti-GRB7 (IP:10045-1-Ig, 3ug; Detection:10045-1-Ig 1:500) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GRB7 antibody (10045-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10045-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA GRB7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRB7 fusion protein Ag0023
• Vollständiger Name: growth factor receptor-bound protein 7
• Berechnetes Molekulargewicht: 532 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC006535
• Gene symbol: GRB7
• Gene ID (NCBI): 2886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.

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