GREB1 Polyklonaler Antikörper

GREB1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28699-1-AP

Synonyme

GREB1, GREB1 protein, KIAA0575, Protein GREB1

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  WB analysis using 28699-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28699-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 28699-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 28699-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28699-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 28699-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28699-1-AP bindet in WB, IHC, IF/ICC, ELISA GREB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GREB1 fusion protein Ag29908
• Vollständiger Name: GREB1 protein
• Berechnetes Molekulargewicht: 216 kDa
• Beobachtetes Molekulargewicht: 216-240 kDa
• GenBank-Zugangsnummer: NM_014668
• Gene symbol: GREB1
• Gene ID (NCBI): 9687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.

Publikationen

Species	Application	Title
		Cancer Gene Ther; Tafazzin mediates tamoxifen resistance by regulating cellular phospholipid composition in ER-positive breast cancer; Authors - Xuan Li
human	WB	Cancers (Basel); Pharmacological Inhibition of CBP/p300 Blocks Estrogen Receptor Alpha (ERα) Function through Suppressing Enhancer H3K27 Acetylation in Luminal Breast Cancer.; Authors - Aaron Waddell
human	WB	Exp Cell Res; EFHD1 promotes breast cancer progression through estrogen signaling; Authors - Xiaojun Sun