GREB1 Polyklonaler Antikörper

GREB1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28699-1-PBS

Synonyme

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  WB analysis using 28699-1-AP (same clone as 28699-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 28699-1-AP (same clone as 28699-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28699-1-AP (same clone as 28699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 28699-1-AP (same clone as 28699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 28699-1-AP (same clone as 28699-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 28699-1-AP (same clone as 28699-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28699-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GREB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GREB1 fusion protein Ag29908
• Vollständiger Name: GREB1 protein
• Berechnetes Molekulargewicht: 216 kDa
• Beobachtetes Molekulargewicht: 216-240 kDa
• GenBank-Zugangsnummer: NM_014668
• Gene symbol: GREB1
• Gene ID (NCBI): 9687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.