GREB1 Rekombinanter Antikörper

GREB1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242882D11

Kat-Nr. : 85189-4-RR

Synonyme

242882D11, GREB1 protein, KIAA0575, Protein GREB1

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  WB analysis of MCF-7 using 85189-4-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 85189-4-RR

  T-47D cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: T-47D-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85189-4-RR bindet in WB, ELISA GREB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GREB1 fusion protein Ag29908
• Vollständiger Name: GREB1 protein
• Berechnetes Molekulargewicht: 216 kDa
• Beobachtetes Molekulargewicht: 216-240 kDa
• GenBank-Zugangsnummer: NM_014668
• Gene symbol: GREB1
• Gene ID (NCBI): 9687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.