Glutamate receptor 3/GluA3 Polyklonaler Antikörper

Glutamate receptor 3/GluA3 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29588-1-AP

Synonyme

GRIA3, GluA3, GluR 3, GLUR C, GLUR K3

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  WB analysis using 29588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (GRIA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 29588-1-AP

  IP result of anti-GRIA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of mouse brain using 29588-1-AP

  IP result of anti-Glutamate receptor 3/GluA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 29588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 29588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamate receptor 3/GluA3 antibody (29588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 29588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Glutamate receptor 3/GluA3 antibody (29588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

29588-1-AP bindet in WB, IHC, IF-P, IP, ELISA Glutamate receptor 3/GluA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glutamate receptor 3/GluA3 fusion protein Ag30898
• Vollständiger Name: glutamate receptor, ionotrophic, AMPA 3
• Berechnetes Molekulargewicht: 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: NM_007325
• Gene symbol: GRIA3
• Gene ID (NCBI): 2892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA3 (Glutamate ionotropic receptor AMPA type subunit 3), also known as Glutamate receptor R3 or A3 (GluR3 or GluA3), is associated with X-linked intellectual disability (ID), dysmorphic features, and non-syndromic epilepsy(PMID: 32977175). GRIA3 also plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1(PMID: 20689760).

Publikationen

Species	Application	Title
mouse	WB	Cell Discov; Postsynaptic lncRNA Sera/Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC; Authors - Ling-Shuang Zhu
mouse	WB	Brain Res; Low-dose diazepam improves cognitive function in APP/PS1 mouse models: Involvement of AMPA receptors; Authors - Junwen Chen
mouse	IHC	iScience; The decreased astrocyte-microglia interaction reflects the early characteristics of Alzheimer's disease; Authors - Kefu Liu