GRIM19 Monoklonaler Antikörper

GRIM19 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A10C5

Kat-Nr. : 67414-1-PBS

Synonyme

NDUFA13, CI B16.6, CGI-39, CGI 39, Cell death regulatory protein GRIM-19

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  WB analysis of LNCaP using 67414-1-Ig (same clone as 67414-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67414-1-Ig (same clone as 67414-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67414-1-Ig (same clone as 67414-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67414-1-Ig (same clone as 67414-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67414-1-Ig (same clone as 67414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67414-1-Ig (same clone as 67414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50735-1

  Cytometric bead array standard curve of MP50735-1, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-2-PBS. Detection antibody: 67414-1-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50735-3

  Cytometric bead array standard curve of MP50735-3, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-1-PBS. Detection antibody: 67414-4-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67414-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA GRIM19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRIM19 fusion protein Ag20855
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
• Berechnetes Molekulargewicht: 16.5 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC009189
• Gene symbol: GRIM19
• Gene ID (NCBI): 51079
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).