NMDAR1/GRIN1 Polyklonaler Antikörper

NMDAR1/GRIN1 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 27676-1-AP

Synonyme

GRIN1, NMDAR1, GluN1, Glutamate receptor ionotropic, NMDA 1, NMDAR 1

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  WB analysis using 27676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27676-1-AP

  IP result of anti-NMDAR1/GRIN1 (IP:27676-1-AP, 4ug; Detection:27676-1-AP 1:2000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR1/GRIN1 antibody (27676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 27676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NMDAR1/GRIN1 antibody (27676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

27676-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA NMDAR1/GRIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26364
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_000832
• Gene symbol: NMDAR1
• Gene ID (NCBI): 2902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
rat	WB	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
mouse	WB	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
mouse	WB	J Neurosci; Cortex-specific Tmem169 Deficiency Induces Defects in Cortical Neuron Development and Autism-like Behaviors in Mice; Authors - Junhao Wang
mouse	WB	Exp Neurol; DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice; Authors - Fen Zhuge
rat	WB,IP	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang