NMDAR1/GRIN1 Monoklonaler Antikörper

NMDAR1/GRIN1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1H2C2

Kat-Nr. : 67717-1-Ig

Synonyme

GRIN1, NMDAR1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1, GluN1

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  WB analysis using 67717-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit cerebellum using 67717-1-Ig

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 67717-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67717-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67717-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NMDAR1/GRIN1 antibody (67717-1-Ig, Clone: 1H2C2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Hühnerhirngewebe, Maushirngewebe, Kaninchen-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

67717-1-Ig bindet in WB, IHC, IF-P, ELISA NMDAR1/GRIN1 und zeigt Reaktivität mit Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Huhn, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26364
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 105-120 kDa
• GenBank-Zugangsnummer: NM_000832
• Gene symbol: NMDAR1
• Gene ID (NCBI): 2902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Exp Brain Res; The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults; Authors - Bei Guo
mouse		Heliyon; A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice; Authors - Hanyu Luo
mouse	IF	Biomedicines; D-Serine May Ameliorate Hippocampal Synaptic Plasticity Impairment Induced by Patients' Anti-N-methyl-D-aspartate Receptor Antibodies in Mice; Authors - Hanyu Luo
rat	WB	Biomedicines; Impaired Response to Mismatch Novelty in the Li2+-Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs; Authors - Carlos Nascimento