NMDAR2A/GRIN2A Rekombinanter Antikörper

NMDAR2A/GRIN2A Rekombinant Antikörper für WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240445D4

Kat-Nr. : 83465-2-PBS

Synonyme

GRIN2A, NMDAR2A, 240445D4, glun2a, Glutamate [NMDA] receptor subunit epsilon-1

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  WB analysis using 83465-2-RR (same clone as 83465-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83465-2-RR (GRIN2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83465-2-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83465-2-RR (same clone as 83465-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (83465-2-RR, Clone: 240445D4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83465-2-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83465-2-RR (same clone as 83465-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (83465-2-RR, Clone: 240445D4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83465-2-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83465-2-RR (same clone as 83465-2-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Grin2a Recombinant Antibody (83465-2-RR, Clone:240445D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83465-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00461-2

  Cytometric bead array standard curve of MP00461-2, GRIN2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83465-4-PBS. Detection antibody: 83465-2-PBS. Standard: Ag29101. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83465-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83465-2-RR against Human NMDAR2A/GRIN2A were performed. The affinity constant is 11.0 nM.

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Geprüfte Anwendungen

Produktinformation

83465-2-PBS bindet in WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA NMDAR2A/GRIN2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NMDAR2A/GRIN2A fusion protein Ag29101
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Berechnetes Molekulargewicht: 165 kDa
• Beobachtetes Molekulargewicht: 160-180 kDa
• GenBank-Zugangsnummer: NM_000833
• Gene symbol: GRIN2A
• Gene ID (NCBI): 2903
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.