NMDAR2B/GRIN2B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NMDAR2B/GRIN2B Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

78

Kat-Nr. : 21920-1-AP

Synonyme

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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Galerie der Validierungsdaten

• 

  WB analysis using 21920-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21920-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21920-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21920-1-AP

  IP Result of anti-NMDAR2B/GRIN2B (IP:21920-1-AP, 3ug; Detection:21920-1-AP 1:2000) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of mouse brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 21920-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 21920-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug NMDAR2B/GRIN2B antibody (21920-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 73 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

21920-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA NMDAR2B/GRIN2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, zebrafink
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMDAR2B/GRIN2B fusion protein Ag16318
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Berechnetes Molekulargewicht: 1484 aa, 166 kDa
• Beobachtetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: BC113620
• Gene symbol: GRIN2B
• Gene ID (NCBI): 2904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publikationen

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury.; Authors - Yingwu Shi
mouse	WB	Sci Adv; Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission; Authors - Wenquan Liang
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
human	WB,CoIP	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
mouse	WB,IF,IP	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach

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