NMDAR2B/GRIN2B Monoklonaler Antikörper

NMDAR2B/GRIN2B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1C5E12

Rezensionen (1)

Kat-Nr. : 66565-1-Ig

Synonyme

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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  WB analysis of mouse brain using 66565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66565-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66565-1-Ig bindet in WB, ELISA NMDAR2B/GRIN2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NMDAR2B/GRIN2B fusion protein Ag16718
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Berechnetes Molekulargewicht: 1484 aa, 166 kDa
• Beobachtetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: BC113620
• Gene symbol: GRIN2B
• Gene ID (NCBI): 2904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publikationen

Species	Application	Title
rat	WB	Neuron; Structural basis for channel gating and blockade in tri-heteromeric GluN1-2B-2D NMDA receptor; Authors - Hyunook Kang
mouse	WB	Philos Trans R Soc Lond B Biol Sci; Cage effects on synaptic plasticity and its modulation in a mouse model of fragile X syndrome; Authors - Rasa Volianskis

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is not very strong and specific on hiPSCs derived neurons: it is detectable with an exposure of about 90 seconds, but sometimes gives aspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons