GRK1 Polyklonaler Antikörper

GRK1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24606-1-AP

Synonyme

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

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  WB analysis of Y79 using 24606-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 24606-1-AP (GRK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 24606-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 24606-1-AP (GRK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 24606-1-AP

  IP result of anti-GRK1 (IP:24606-1-AP, 4ug; Detection:24606-1-AP 1:500) with Y79 cells lysate 1240ug.

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  IHC staining of mouse eye using 24606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24606-1-AP (GRK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 24606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24606-1-AP (GRK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 24606-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using GRK1 antibody (24606-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Maus-Augengewebe
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

24606-1-AP bindet in WB, IHC, IF-P, IP, ELISA GRK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRK1 fusion protein Ag18063
• Vollständiger Name: G protein-coupled receptor kinase 1
• Berechnetes Molekulargewicht: 563 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC140945
• Gene symbol: GRK1
• Gene ID (NCBI): 6011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.

Publikationen

Species	Application	Title
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li
mouse	IHC	Zool Res; Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations.; Authors - Zhi-Lin Ren
zebrafish	WB	Biochim Biophys Acta Mol Basis Dis; Knockout of DLIC1 leads to retinal cone degeneration via disturbing Rab8 transport in zebrafish; Authors - Jing Zhang
mouse	WB,IHC	Vision Res; Lack of retinal degeneration in a Dram2 knockout mouse model; Authors - Kuanxiang Sun