GRK1 Rekombinanter Antikörper

GRK1 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

250433A4

Kat-Nr. : 85942-1-RR

Synonyme

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

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  WB analysis using 85942-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse retina using 85942-1-RR

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 85942-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 85942-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 85942-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using GRK1 antibody (85942-1-RR, Clone: 250433A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85942-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85942-1-RR against Human GRK1 were performed. The affinity constant is 3.77 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, Maus-Retina-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85942-1-RR bindet in WB, IHC, IF-P, ELISA GRK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRK1 fusion protein Ag18063
• Vollständiger Name: G protein-coupled receptor kinase 1
• Berechnetes Molekulargewicht: 563 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC140945
• Gene symbol: GRK1
• Gene ID (NCBI): 6011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.