GRK2 Polyklonaler Antikörper

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• KD/KO Validated

GRK2 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13990-1-PBS

Synonyme

ARBK1, ADRBK1, BETA ARK1, Beta ARK 1, BARK1

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  WB analysis using 13990-1-AP (same clone as 13990-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13990-1-AP (GRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 13990-1-AP (same clone as 13990-1-PBS)

  IP result of anti-GRK2 (IP:13990-1-AP, 4ug; Detection:13990-1-AP 1:1000) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13990-1-AP (same clone as 13990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13990-1-AP (same clone as 13990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13990-1-AP (same clone as 13990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 13990-1-AP (same clone as 13990-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug GRK2 Polyclonal antibody (13990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13990-1-PBS bindet in WB, IHC, FC (Intra), IP, Indirect ELISA GRK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRK2 fusion protein Ag5071
• Vollständiger Name: adrenergic, beta, receptor kinase 1
• Berechnetes Molekulargewicht: 689 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC037963
• Gene symbol: ADRBK1
• Gene ID (NCBI): 156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)