GRK5 Polyklonaler Antikörper

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• KD/KO Validated

GRK5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17032-1-PBS

Synonyme

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  WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17032-1-PBS bindet in WB, IHC, Indirect ELISA GRK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRK5 fusion protein Ag10511
• Vollständiger Name: G protein-coupled receptor kinase 5
• Berechnetes Molekulargewicht: 590 aa, 68 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC064506
• Gene symbol: GRK5
• Gene ID (NCBI): 2869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry.