GRN Rekombinanter Antikörper

GRN Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250681G12

Kat-Nr. : 86118-1-PBS

Synonyme

granulin, Epithelin precursor, Glycoprotein 88, Glycoprotein of 88 Kda, Granulin precursor

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  Sandwich ELISA standard curve of MP02283-1

  Sandwich ELISA standard curve of MP02283-1, Human Progranulin/PGRN Recombinant Matched Antibody Pair - PBS only. 86118-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2954. 86118-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP02283-1

  The mean Progranulin/PGRN concentration was determined to be 64.7 ng/mL in human serum.

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  Sample test of MP02283-1

  Plasma of eight individual healthy human donors was measured. The human Progranulin/PGRN concentration of detected samples was determined to be 37.53 ng/mL with a range of 27.63 - 51.07 ng/mL.

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  Sample test of MP02283-1

  Human milk of eight individual healthy human donors was measured. The human Progranulin/PGRN concentration of detected samples was determined to be 145.13 ng/mL with a range of 57.37 - 213.91 ng/mL.

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  Sample test of MP02283-1

  Saliva of eight individual healthy human donors was measured. The human Progranulin/PGRN concentration of detected samples was determined to be 58.42 ng/mL with a range of 18.53 - 104.43 ng/mL.

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  Sample test of MP02283-1

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate wasremoved, assayed for human Progranulin/PGRN, and measured 7.82 ng/mL.

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  Cytometric bead array standard curve of MP02283-1

  Cytometric bead array standard curve of MP02283-1, Progranulin/PGRN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86118-2-PBS. Detection antibody: 86118-1-PBS. Standard: Eg2954. Range: 0.625-80 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86118-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test GRN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRN fusion protein Eg2954
• Vollständiger Name: granulin
• Berechnetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: NM_002087.3
• Gene symbol: Granulin
• Gene ID (NCBI): 2896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.