Progranulin/PGRN Rekombinanter Antikörper

Progranulin/PGRN Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250681G2

Kat-Nr. : 86118-2-PBS

Synonyme

granulin, Epithelin precursor, Glycoprotein 88, Glycoprotein of 88 Kda, Granulin precursor

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  WB analysis using 86118-2-RR (same clone as 86118-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86118-2-RR (GRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86118-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02283-1

  Sandwich ELISA standard curve of MP02283-1, Human Progranulin/PGRN Recombinant Matched Antibody Pair - PBS only. 86118-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2954. 86118-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

• 

  Sample test of MP02283-1

  The mean Progranulin/PGRN concentration was determined to be 64.7 ng/mL in human serum.

• 

  Sample test of MP02283-1

  Plasma of eight individual healthy human donors was measured. The human Progranulin/PGRN concentration of detected samples was determined to be 37.53 ng/mL with a range of 27.63 - 51.07 ng/mL.

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  Sample test of MP02283-1

  Human milk of eight individual healthy human donors was measured. The human Progranulin/PGRN concentration of detected samples was determined to be 145.13 ng/mL with a range of 57.37 - 213.91 ng/mL.

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  Sample test of MP02283-1

  Saliva of eight individual healthy human donors was measured. The human Progranulin/PGRN concentration of detected samples was determined to be 58.42 ng/mL with a range of 18.53 - 104.43 ng/mL.

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  Sample test of MP02283-1

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate wasremoved, assayed for human Progranulin/PGRN, and measured 7.82 ng/mL.

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  Cytometric bead array standard curve of MP02283-1

  Cytometric bead array standard curve of MP02283-1, Progranulin/PGRN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86118-2-PBS. Detection antibody: 86118-1-PBS. Standard: Eg2954. Range: 0.625-80 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86118-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Progranulin/PGRN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Progranulin/PGRN fusion protein Eg2954
• Vollständiger Name: granulin
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: NM_002087.3
• Gene symbol: Granulin
• Gene ID (NCBI): 2896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.