GRP75 Monoklonaler Antikörper

GRP75 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B12F2

Kat-Nr. : 67563-1-PBS

Synonyme

HSPA9, 2B12F2, 75 kDa glucose-regulated protein, CSA, GRP-75

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  WB analysis using 67563-1-Ig (same clone as 67563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67563-1-Ig (GRP75 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67563-1-PBS bindet in WB, IHC, Indirect ELISA GRP75 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRP75 fusion protein Ag7125
• Vollständiger Name: heat shock 70kDa protein 9 (mortalin)
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC000478
• Gene symbol: GRP75
• Gene ID (NCBI): 3313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)