GRP75 Rekombinanter Antikörper

GRP75 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251670D1

Kat-Nr. : 86721-1-RR

Synonyme

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9

---

• 

  WB analysis using 86721-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86721-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, HT-29-Zellen, Mauslebergewebe, Maushodengewebe, NIH/3T3-Zellen, SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86721-1-RR bindet in WB, ELISA GRP75 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRP75 fusion protein Ag6673
• Vollständiger Name: heat shock 70kDa protein 9 (mortalin)
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC000478
• Gene symbol: GRP75
• Gene ID (NCBI): 3313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)